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Test Code FLURSVCOV Influenza RSV COVID PCR

Infectious

Collection Method

Nasopharyngeal swab

Container

NPSWAB

Instructions/Special Handling

  • Tilt patient’s head back 70 degrees.
  • Gently and slowly insert a minitip swab with a flexible shaft (wire or plastic) through the nostril parallel to the palate (not upwards) until resistance is encountered or the distance is equivalent to that from the ear to the nostril of the patient, indicating contact with the nasopharynx.
  • Gently rub and roll the swab.
  • Leave swab in place for several seconds to absorb secretions.
  • Slowly remove swab while rotating it. Specimens can be collected from both sides using the same swab, but it is not necessary to collect specimens from both sides if the minitip is saturated with fluid from the first collection.
  • If a deviated septum or blockage create difficulty in obtaining the specimen from one nostril, use the same swab to obtain the specimen from the other nostril.
  • Place swab, tip first, into the transport tube provided.

Preferred Specimen

NPSWAB

Acceptable Specimes

NPSWAB

Units

N/A

Reference Ranges

SARS-CoV-2 POSITIVE

The SARS-CoV-2 target RNA is detected

• TheSARS-CoV-2 target has a Ct within the valid range and endpoint above the minimum setting.

• SPC-NA; SPC is ignored because the SARS-CoV-2 target amplification occurred

• Probe Check-PASS; all probe check results pass.
Flu A POSITIVE

The Flu A signal for either the Flu A1 RNA target or the Flu A2 RNA target has a Ct within the valid range and endpoint above the threshold setting.

• SPC-NA; SPC is ignored because Flu A target amplification occurred

• Probe Check-PASS; all probe check results pass.
Flu B POSITIVE

The Flu B target has a Ct within the valid range and endpoint above the minimum setting.

• SPC-NA; SPC is ignored because Flu B target amplification occurred

• Probe Check-PASS; all probe check results pass
RSV POSITIVE

The RSV Target RNA is detected.

• The RSV signal has a Ct within the valid range and endpoint above the minimum setting.

• SPC-NA; SPC is ignored because RSV target amplification occurred.

• Probe Check-PASS; all probe check results pass.
SARS-CoV-2 NEGATIVE, Flu A NEGATIVE, Flu B NEGATIVE, RSV NEGATIVE

SARS-CoV-2 target RNA is not detected; Flu A target RNA is not detected; Flu B target RNA is not detected; RSV target RNA is not detected.

• SARS-CoV-2, Flu A, Flu B and RSV target RNAs are not detected

• SPC-PASS; SPC has a Ct within the valid range and endpoint above the minimum setting.

• Probe Check-PASS; all probe check results pass.
INVALID

SPC does not meet acceptance criteria and all targets are not detected. Repeat test according to the instructions in this procedure

• SPC-FAIL; SPC and SARS-CoV-2, Flu A, Flu B and RSV RNA signals do not have a Ct within valid range and endpoint is below minimum setting.

• Probe Check-PASS; all probe check results pass.
ERROR

Presence or absence of SARS-CoV-2, Flu A, Flu B and RSV target RNA cannot be determined. Repeat test according to the instructions in this procedure.

• SARS-CoV-2-NO RESULT

• Flu A-NO RESULT

• Flu B-NO RESULT

• SPC-NO RESULT

• Probe Check-FAIL* ; all or one of the probe check results fail. * If the probe check passed, the error is caused by the maximum pressure limit exceeding the acceptable range or by a system component failure.
NO RESULT

Presence or absence of SARS-CoV-2, Flu A, Flu B or RSV target RNA cannot be determined. Repeat test according to the instructions in this procedure. A NO RESULT indicated that insufficient data were collected. For example, the operator stopped a test that was in progress or a power failure occurred.

• SARS-CoV-2-NO RESULT

• Flu A-NO RESULT

• Flu B-NO RESULT

• SPC-NO RESULT

• Probe Check-NA

 

 

 

 

Critical Values

N/A

Reasons for Rejection

 

Hemolysis N/A
Icterus N/A
Lipemia N/A
Other QNS, Contaminated, Improperly labeled, Improper specimen type

Specimen Stability

Ambient (15-25°C) 8 hours
Refrigerated (2-8°C) 7 days
Frozen (-20°C) N/A

 

Performing Site

Boone Health Laboratory

Performance Information

Days/Times Performed Daily 24/7
Expected Turn around Time Same day
Methodology/Method Description Cepheid

CPT Code(s)

0241U